SHC Flow Cytometry Core
Affiliated with University of Cincinnati
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Facility details:
This core provides a range of flow cytometry services, including assistance with cell analysis and cell sorting. By using this facility you agree to acknowledge the work produced at SHC Flow Cytometry Core Facility in presentations and publications, details on how to do this are at the bottom of this page.
First time users
First time users will need to contact us to consult with core staff for training on appropriate instruments. Once trained users are able to book time and access the available instruments they have been trained on.
Regular core users
Regular core users are able to view the schedule here, or contact us for booking time on instruments.
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Facility staff are available to assist you with acquisition, analysis, cell sorting, or to answer any questions you may regarding the instruments or the facility. The facility core runs on a cost recovery basis.  Pricing is based on instrument capabilities. The cost is listed below. For further information please contact us.

Instrumentation available on site:

Coulter XL: Cell analyzer, able to analyse 2 scatter and up to 4 fluorescence parameters using a 488nm (blue) excitation source.
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Cost: $30 per hour
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With unique flow cell design and sensitivity, this instruments is ideal for:
* DNA / Cell cycle analysis
* Microparticle studies
BD LSRII:Cell analyzer, able to analyze 2 scatter and up to 18 fluorescence parameters  using a combination of 355nm (UV), 405nm (violet), 488nm (blue), 553nm (yellow/green)and 640nm (red) excitation sources.
A removable High Throughput Sampler (HTS) is also available for analysis of 96 and 384 well plates.
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Cost: $35 per hour
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With a large number of parameters, this instrument is ideal for:
* Immunophenotyping
* Mulitcolor analysis
 
FACS Vantage SE (celll corter with Diva option): Cell sorter, able to analyze and sort cells based on 2 scatter and up to 10 fluorescence parameters, using a combination of 488nm (blue), 640nm (red), and 405nm (violet) excitation sources. Up to 4 subpopulations may be sorted simultaneously in a range of collection tubes or 96 well plate. Single cell sorting is also possible.
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Cost: $70 per hour
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With enchanced optics, and upgraded electronics,
this instrument is ideal for:
* Transfection purification
* Single cell sorting
* Multicolor subset purification
Minimum charge for all instruments is 30 minutes, cancellations with less than 24 hours notice will be charged in full for time booked.
There is no cost for set-up or assistance with sample acquisition, or data analysis. However by using the core you agree to acknowledge the services provided by SHC Flow Cytometry Core Facility and staff in presentations and publications.
To cite us:
Flow cytometry was performed at Shriners Hospitals for ChildrenTM - Cincinnati, supported by a grant from the Shriners of North America SSF 84070
If detailed assistance is needed for protocol design and/or complex data analysis is provided by the core, then authorship will be agreed.