SHC Flow Cytometry Core
Affiliated with University of Cincinnati
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Facility details:
This core provides a range of flow cytometry services, including assistance with cell analysis and cell sorting. By using this facility you agree to acknowledge the work produced at SHC Flow Cytometry Core Facility in presentations and publications, details on how to do this are at the bottom of this page.
First time users
First time users will need to consult with core staff regarding project prior to scheduling appointments.
Regular core users
Regular core users are able to view the schedule for booking time on instruments.
Facility staff are available to assist you with acquisition, analysis, cell sorting, or to answer any questions you may regarding the instruments or the facility. The facility core runs on a cost recovery basis.  Pricing is based on instrument capabilities. The cost is listed below. 

Instrumentation available on site:

Coulter XL: Cell analyzer, able to analyse 2 scatter and up to 4 fluorescence parameters using a 488nm (blue) excitation source.
Cost: $30 per hour, billed in 30 minute increments
Potential use: DNA / Cell cycle analysis

BD LSRII:Cell analyzer, able to analyze 2 scatter and up to 18 fluorescence parameters  using a combination of 355nm (UV), 405nm (violet), 488nm (blue), 553nm (yellow/green)and 640nm (red) excitation sources.
A removable High Throughput Sampler (HTS) is also available for analysis of 96 and 384 well plates.
Cost: $35 per hour, billed in 30 minute increments
Potential use: Mulitcolor analysis

COMING SOON:  MoFlo Astrios EQ: Cell sorter, able to analyze and sort cells based on 2 scatter and up to 19 fluorescence parameters, using a combination of 488nm (blue), 640nm (red), 405nm (violet), 561nm (green) and 355nm (UV) excitation sources. Up to 6 subpopulations may be sorted simultaneously into 5ml tubes. Additional collection options include 2- 50ml or 2-15ml tubes and 6, 24, 96, 384, and 1536 well plates.  
The Astrios has a second FSC parameter that can potentially enhance the evaluation of small particles.
Cost:  $75 per hour, billed in 30 minute increments  plus a $35 set up fee.
Potential uses:  Single Cell Sorting
                       Multicolor subset purification
                       Transfection purification
***Appointment cancellations with less than 24 hours notice will be charged a $35 instrument setup fee. ***  Cancellations must be submitted by email.

By using the core you agree to acknowledge the services provided by SHC Flow Cytometry Core Facility and staff in presentations and publications.
To cite us:  Flow cytometry was performed at Shriners Hospitals for ChildrenTM - Cincinnati, supported by a grant from the Shriners of North America SSF 84070

If detailed assistance is needed for protocol design and/or complex data analysis is provided by the core, then authorship will be agreed.