SHC Flow Cytometry Core
Affiliated with University of Cincinnati
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Flow Cytometry intro:
 
 
Flow cytometry is a unique tool for analyzing cells based on physical and chemical properties. One can use light scatter properties and a variety of fluorescent labels  to characterize cells populations and isolate individual populations for downstream needs. A number of applications are available, including:
 
* Multicolor analysis: The ability to simultaneously measure numerous parameters of a single cell and is one of the most powerful features about flow cytometry. For example, one may measure the presence or absence of several different proteins on a cell surface for cell phenotyping.  
 
* Cell DNA / cell cycle analysis: Provides information about the cell cycle and effects of treatment and/or stimuli on cell proliferation. When combined with particular markers it is possible to look at sub-sets of cells or assess the level of a given marker at different stages of the cell cycle. 
 
* Systems biology: This includes monitoring signalling pathways for intracellular protein phosphorylation events to detecting rare sub-populations of cells by uncovering alternative signaling mechanisms or targeting drug effects  
 
* Functional studies: An increasing array of probes are available for assessing functionality, including monitoring apoptosis, determining cell viability, monitoring calcium flux mobilization, and investigating cell proliferation, and are common flow cytometry protocols.
 
*Microbial applications: By monitoring rapid bacterial enumeration through to live/dead viability assay on microbial samples, flow cytometry has many benefits over traditional microbiology methods.
 
*Cell sorting: This feature enables isolation and purification of populations of cells for culturing, or single cell sorting for clonal studies and downstream applications. Cell sorting is particularly relevant for purifying transfected cells, where only a small portion of the cells may be expressing the target protein of interest. Sorting may be performed based on fluorescent protein expression, antigen expression, DNA content, cell function, light scatter properties or a combination of these.
 
*Microparticle study: This is a "hot" topic among many researchers. Developing protocols to analyze, characterize, and isolate microparticles is a challenge that is ongoing in the flow cytometry community.  
There are many more applications possible using flow cytometry, and with the instrumentation and expertise available at SHC Flow Core there may be a way in which flow cytometry can support your research, so please contact us for further information or advice with how we may be able to assist you.
 
If you have any further questions please contact us.